What is the procedure of one-time lumbar puncture needle?

Usually, the patient was placed in the bent lateral position, and intervertebral puncture was performed from the 2nd to the 1st sacral (mainly the 3rd to 4th lumbar). After local routine disinfection and anesthesia, wear rubber gloves and slowly Pierce the needle with No. 20 (No. 21-22 for children) along the direction of spinous process. When the needle tip encounters bone during the process of needle insertion, the needle should be returned to the subcutaneous Angle to be corrected before puncture. When the injection is about 4-6cm for adults (about 3-4cm for children), the dura dura can be punctured and the subnet cavity of the spider membrane can be reached. The cerebrospinal fluid can be drained out after the needle core is extracted. After pressure measurement and slow release of the fluid (no more than 2-3ml), the needle core can be inserted and the puncture needle can be pulled out. The puncture site was slightly pressurized to stop the bleeding, applied with sterile gauze and fixed with tape. Lie supine for 4-6 hours after surgery. If the initial pressure exceeds 2.94kPa(300mm water column), it is not suitable to discharge fluid. Only take cerebrospinal fluid from the pressure measuring tube and send it to cell count and protein quantition.