Microbiological examination of Legionella pneumophila

Generally, specimens such as lower respiratory tract secretions, lung biopsy tissue or prepleural effusion are taken for bacteriological examination. Bacteria were isolated with BCYE medium, and then identified according to the characteristics of pneumonia, colony characteristics, and biochemical reactions, but the results often appeared later. The specimen can be used for direct fluorescence experiment with a fluorescently labeled antibody, which is both specific and can make a rapid diagnosis. Tests such as ELISA, RIA and latex agglutination can be used to detect the specific antigen of the bacteria in the specimen or use PCR technology to check the nucleic acid of the bacteria for rapid diagnosis.

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