Why can't the needle be inserted into the bottom of the bottle

Puncture and inoculation should be conducted as vertically as possible. Touching the bottom may cause the needle to shake and disturb the puncture line, which may affect observation, or cause bacteria to spread from the AGAR to the glass tube and affect observation.

However, this note is not very strict, even if accidentally touched the bottom of the general can clearly distinguish the negative or positive power.

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