What is the examination process of bone marrow aspiration?

Jan 03, 2022

Routinely disinfect the skin, wear sterile gloves, spread a sterile hole towel, and use 2% lidocaine for local infiltration anesthesia until the periosteum. Fix the bone marrow puncture needle holder at an appropriate length (the iliac bone puncture is about 1.5 cm, and the obese people can lengthen it appropriately, and the sternal stem puncture is about 1.0 cm). Use the left thumb and index finger to fix the skin of the puncture site, and hold the needle on the bone surface with the right hand. Vertical stabbing. When the puncture needle touches the bone, it rotates left and right to slowly drill the bone. When the resistance disappears and the puncture needle is fixed in the bone, it means that it has entered the bone marrow cavity. Pull out the needle core, connect a dry 20mL syringe, and slowly suck with appropriate strength. It can be seen that a small amount of red bone marrow fluid enters the syringe. The suction volume of bone marrow fluid is preferably 0.1~0.2 mL. Remove the syringe and push the bone marrow fluid. On the glass slide, the assistant quickly makes 5 to 6 smears, and submits them for cell morphology and cytochemical staining. If bone marrow culture is needed, connect the syringe and suck 2~3 mL of bone marrow fluid into the culture medium. After the suction is completed, insert the needle core, turn slightly to pull out the puncture needle, then cover the needle hole with sterile gauze, press it slightly, and fix it with tape.

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