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Usually take the side-bending position, puncture the intervertebral space from lumbar 2 to sacrum 1 (mainly lumbar 3-4). After local routine disinfection and anesthesia, wear rubber gloves and slowly pierce in the direction of the spinous process with a No. 20 puncture needle (No. 21-22 for children). When the needle tip encounters bone during the needle insertion process, the needle should be retracted under the skin. Perform the puncture after correcting the angle. When the needle is inserted about 4-6cm in adults (about 3-4cm in children), the dura mater can be penetrated to reach the subarachnoid space, and the needle core will flow out of the cerebrospinal fluid. After pressure measurement and slow discharge (no more than 2-3ml) ), then insert the needle core and pull out the puncture needle. Slightly pressurize the puncture point to stop bleeding, apply sterile gauze and fix it with tape. Lie supine for 4-6 hours postoperatively. If the initial pressure exceeds 2.94kPa (300mm water column), it is not suitable to discharge the liquid, just take the cerebrospinal fluid in the piezometer tube for cell count and protein quantification.

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