How to make a embryo after egg retrieval
Apr 21, 2022
production of female eggs
A woman's egg production is clinically called controlled superovulation and takes 10 to 15 days.
A healthy woman generally has only one dominant follicle in the natural ovulation cycle (sometimes there are more than one, that is, fraternal twins). The egg in this dominant follicle matures, ovulates, and can only form an embryo after fertilization.
It can be seen that the general natural ovulation is 1. In vitro fertilization with one egg can only produce one embryo at most. There is no choice when it comes to embryo transfer. The pregnancy rate for transferring 1 embryo is very low. Sometimes, the egg may not be fertilized or developed, and there is no chance of pregnancy. Therefore, it is necessary to use controlled superovulation to enhance and improve the function, so as to achieve the purpose of obtaining multiple healthy eggs without being restricted by the natural cycle, so that more embryos can be cultivated in the laboratory, and high-quality embryos can be selected for transfer. , can increase the chance of pregnancy.
Egg induction maturation (35-36 hours before egg retrieval)
The above-mentioned use can only make the eggs grow in most cases, but cannot obtain mature eggs. To assess whether the egg in the follicle has the ability to mature, the doctor must monitor the size of the follicle with an ultrasound machine and check the blood level. When 2 or 3 or more follicles are larger than 1.8 to 2 cm in diameter and the level is consistent with the number of follicles, then the eggs have the ability to mature. Doctors can use human chorionic gonadotropin to stimulate the eggs in the follicles to mature. The maturation time of human eggs is about 36-40 hours, and the human villous gonadotropin must be retrieved within 35-36 hours. Therefore, the doctor will schedule the egg retrieval time.
Egg retrieval (Embryos Day 0)
Egg retrieval is performed within 35 to 36 hours of human chorionic gonadotropin. Early or late egg retrieval can affect the egg retrieval rate and the quality of the eggs retrieved. Too early may make the eggs not fully mature, there are more immature eggs, and too late may make some large follicles ovulate, so the eggs cannot be retrieved. Egg retrieval is a small procedure that can be done under general anesthesia or under local anesthesia. Guided by a transvaginal ultrasound instrument, the oocyte retrieval needle is passed through the vagina until the eggs are sucked up. The laboratory personnel immediately move the sucked eggs to a petri dish containing embryo culture medium under the microscope and place them in an incubator at 37°C. nourish.
The egg retrieval time is usually within 30 minutes. After egg retrieval, the patient has only mild pain and a very small amount, and can go home after resting for 1 to 2 hours, which will not affect daily work and life.
However, to know their exact stage of maturation, these cells must be separated from the egg. Generally speaking, the following types of eggs can be found:
Mature egg The release of the first polar body is an indicator of egg maturation.
2. Immature eggs
3. Over-mature eggs The eggs must be fertilized after they are mature. 4. Abnormal eggs cannot be used for fertilization.
At this point, the egg retrieval procedure is over, and the doctor will remove the frozen or fresh semen, fertilize it, implant it in the woman who wants to conceive, and finally mature in the mother's uterus. Character limit is for reference.
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