Is the fertility process complicated?

1. Controlled ovulation

Since the length of the natural menstrual cycle varies from person to person, and different cycles of the same patient are also different, it is not easy to arrange the egg retrieval time, and only one dominant follicle develops in the natural cycle, and only one embryo can be formed after fertilization, and one embryo is transferred. The rate is very low, so it is necessary to use controlled superovulation to enhance and improve ovarian function, in order to achieve the goal of getting multiple healthy eggs without being restricted by the natural cycle, providing multiple embryo transfers, and making the corpus luteum develop as much as possible. The endometrial function is synchronized.

2. Monitor follicles

In order to evaluate the effect of ovarian stimulation and determine the time of egg retrieval, vaginal ultrasound must be used to monitor the size of the follicles, along with a blood draw to check the estrogen value, and adjust the dosage of the drug. When the diameter of 2 to 3 follicles is greater than 1.8cm and 1.4cm The number of follicles above is equivalent to the value of estrogen, human chorionic gonadotropin (HCG) can be injected to promote the maturation of the follicles, and the eggs will be retrieved 34 to 36 hours after the injection of hCG.

3. Retrieve the eggs

The more commonly used method of egg retrieval is under local anesthesia, guided by vaginal B-ultrasound, and the needle is inserted through the vaginal fornix to reach the ovary to suck the egg, and immediately move the egg to a petri dish containing embryo culture medium under the microscope Incubate in an incubator at 37°C.

4. Take the essence

The sperm retrieval time is the same day as the egg retrieval day. Use a special sterile cup. Do not touch the rim and the inside of the cup when retaining it. The semen retrieved is processed by the upstream method or the Percoll density gradient centrifugation method.

5. After in vitro fertilization and egg retrieval

Put the treated sperm and egg in the same petri dish for 4 to 5 hours. After 18 hours of co-cultivation, the fertilization can be observed under a microscope. If the quality of the sperm is too poor to be fertilized naturally, the microinjection method must be used Forced fertilization.

6. Embryo culture in vitro

7. Embryo transfer

Fertilized eggs can be cultured in vitro for 48 to 72 hours to develop to 8-16-cell stage embryos. At this time, the number of embryos to be transferred is determined according to the age of the patient, whether they have been pregnant or not, and the quality of the embryos. The excess embryos can be cryopreserved. Embryo transfer generally does not require anesthesia. At present, embryos are usually transferred 2 to 3 days after fertilization, and embryo transfer is used 3 to 5 days after fertilization.

8. After embryo transfer

Supplement progesterone, check the morning urine on the 14th day after transplantation to determine whether the pregnancy is pregnant, and on the 14th day after pregnancy, the number of fetuses and the implantation site of the embryo will be checked by B-ultrasound.

From the above content, it can be seen that the process of assisted pregnancy is not complicated at all, and we only need to follow the specific steps at that time.

If you have any questions, please contact us. Our company can produce various customized needles, medical needles, puncture needles, hypodermic needles, biopsy needles, vaccine needles, injection needles, syringe needles, veterinary needles, pencil point needle, ovum pick up needles, spinal needles, etc. If you need customized needle products, please contact us. We look forward to your inquiry! The quality of the products manufactured in our factory will surely satisfy you!

Please contact us if you need: zhang@sz-manners.com