How is the test tube baby retrieved?

1. Confirm the mature follicles before surgery. After the patient has emptied the bladder, take a cross-sectional lithotomy position, routinely disinfect the vulva and vagina, and apply a towel.

2. Put the sterile vaginal probe into the vagina, detect the ovaries and mature follicles in the fornix, fix the probe on the puncture site, make the mature follicles on the puncture guide line and measure the depth of needle insertion.

3. Under the guidance of vaginal ultrasound, the puncture needle is inserted into the follicle through the vagina and fornix. When the puncture needle reaches the surface of the follicle, after a short pause, moderate force and acceleration should be applied to quickly penetrate the puncture needle into the follicular cavity.

4. Confirm that the needle tip is located in the follicular cavity, turn on the negative pressure suction device, and aspirate the follicular fluid until the follicle collapses. After the follicular fluid of one follicle is drained, the needle is inserted into the adjacent larger follicle. If the number of dominant follicles is less than 4 to 5, or there are many immature follicles, a double-lumen oocyte retrieval needle should be used.

If the number of dominant follicles exceeds 4-5, a single-lumen oocyte retrieval needle can be used. If the number of retrieved eggs is found to be less than the number of follicles seen by ultrasound during the oocyte retrieval process, a double-lumen oocyte retrieval needle should be used instead, and each follicle is treated with culture medium Rinse 2-3 times to get more eggs and avoid loss.

5. Try to puncture all follicles with a diameter greater than 10mm, and the follicles located on the same puncture line can be completed within one needle insertion from shallow to deep. For the follicles on different puncture lines, withdraw the needle to the surface of the ovary, but not the vaginal wall, change the puncture direction and perform puncture again. After the puncture of one side was completed, the other side of the ovary was punctured.

6. At the end of the puncture, withdraw the puncture needle to the outside of the body. Routine scans of the pelvis are performed to check for possible internal bleeding or hematoma formation.

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