How far has IVF progressed so far?

1. Controlled superovulation: Because the length of the natural menstrual cycle varies from person to person, and there are differences in different cycles of the same patient, it is not easy to arrange the time of egg retrieval, and only one dominant follicle develops in the natural cycle, and only one embryo can be formed after fertilization , and the pregnancy rate of transferring one embryo is very low. Therefore, it is necessary to use controlled superovulation to enhance and improve ovarian function, in order to achieve the purpose of obtaining multiple healthy eggs without being restricted by the natural cycle, providing multiple embryo transfers, and synchronizing corpus luteum development with endometrial function as much as possible. Controlled ovarian hyperstimulation generally uses GnRHa to down-regulate FSH and LH in the body, and then administer HMG or FSH ovulation drugs to stimulate follicle growth in the ovary. The number of eggs obtained differs depending on the dose used;

2. Monitoring of follicles: In order to evaluate the effect of ovarian stimulation and determine the time of egg retrieval, the size of the follicles must be monitored by vaginal B-ultrasound, and the E2 value (estrogen) should be checked with blood to adjust the dosage. When two to three or more follicles are larger than 1.8cm in diameter, and the number of follicles over 1.4cm is equivalent to the E2 value, human chorionic gonadotropin (hCG) can be injected to promote follicle maturation. Egg retrieval 34-36 hours after hCG injection;

3. Egg retrieval: The most commonly used method of egg retrieval is under the guidance of local anesthesia, transvaginal B-ultrasound guidance, passing the oocyte retrieval needle through the vaginal fornix, reaching the ovary to extract the eggs, and immediately moving the eggs to the embryo-containing culture under the microscope. In a petri dish of liquid, place it in an incubator at 37 °C;

4. Sperm retrieval: The sperm retrieval time is the same day as the egg retrieval day. Wash hands before semen collection, and use masturbation to collect semen. The small cups given are sterile, do not touch the rim and the inside of the cup when saving. The extracted semen is processed by upstream method or Percoll density gradient centrifugation;

5. In vitro fertilization: 4-5 hours after egg retrieval, the treated sperm and eggs are placed in the same petri dish, and after co-cultivation for 18 hours, the fertilization can be observed under a microscope. If the sperm quality is too poor for natural fertilization, forced fertilization must be performed by microinjection (refer to intracytoplasmic single sperm microinsemination);

6. Embryo culture in vitro

7. Embryo transfer: fertilized eggs can develop to 8-16 cell stage embryos in vitro for 48-72 hours. At this time, the number of embryos to be transferred is determined according to the patient's age, whether it has been pregnant or not, and the quality of the embryos. The excess embryos can be cryopreserved. Embryo transfer generally does not require anesthesia. Delaying the time of embryo transfer requires higher conditions for in vitro culture, but delaying the transfer time is more in line with pregnancy physiology. At the same time, it can also eliminate inferior embryos through natural screening, which can improve the pregnancy rate and reduce the multiple birth rate;

8. Progesterone supplementation after embryo transfer: Progesterone is given by injection to support the corpus luteum. If pregnancy is confirmed, switch to hCG and continue supplementation until 10 weeks of pregnancy. 14 days after embryo transfer, pregnancy can be determined by a urine test or blood draw. 14 days after pregnancy, B-ultrasound to check the number of fetuses and embryo implantation sites.

If you have any questions, please contact us. Our company can produce various customized needles, medical needles, puncture needles, hypodermic needles, biopsy needles, vaccine needles, injection needles, syringe needles, veterinary needles, pencil point needle, ovum pick up needles, spinal needles, etc. If you need customized needle products, please contact us. We look forward to your inquiry! The quality of the products manufactured in our factory will surely satisfy you!

Please contact us if you need: zhang@sz-manners.com