How does IVF get eggs?

1. Confirm the mature follicles before the operation. After the patient empties the bladder, take the cross-section lithotomy position, and routinely disinfect the vulva and vagina and spread towels.

2. Put the sterile vaginal probe into the vagina, detect the ovary and mature follicles in the fornix, fix the probe on the puncture site, place the mature follicle on the puncture guide line and measure the depth of needle penetration.

3. Under the guidance of vaginal ultrasound, the puncture needle penetrates the vagina and fornix to the follicle. When the puncture needle reaches the surface of the follicle, after a short pause, the puncture needle should be quickly pierced into the follicular cavity with moderate force and acceleration.

4. Confirm that the needle tip is located in the follicular cavity, turn on the negative pressure aspirator, and suck the follicular fluid until the follicle collapses. After the follicle fluid of one follicle is exhausted, the needle is inserted into the adjacent larger follicle. If the number of dominant follicles is less than 4~5, or there are many immature follicles, a double-cavity oocyte retrieval needle should be used. If necessary, the follicular cavity can be washed with culture medium and repeated aspiration 2~3 times.

If the number of dominant follicles is more than 4~5, single-chamber egg retrieval needles can be used. If the number of eggs retrieved is less than the number of follicles seen under ultrasound during the egg retrieval process, double-chamber egg retrieval needles should be used instead, with culture medium for each follicle Rinse 2 to 3 times to get more eggs and avoid loss.

5. Try to puncture all follicles with a diameter greater than 10mm. The follicles on the same puncture line can be done from shallow to deep in one needle. For follicles on different puncture lines, withdraw the needle to the surface of the ovary, but without exiting the vaginal wall, change the puncture direction and perform the puncture again. After the puncture on one side is completed, the ovarian puncture on the other side will be performed.

6. At the end of the puncture, withdraw the puncture needle to the body. Routinely scan the pelvic cavity to check for possible internal bleeding or hematoma formation.

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