Can a lumbar puncture be done for sepsis?
Jan 05, 2022
Usually take the side-bending position, puncture the intervertebral space from lumbar 2 to sacrum 1 (mainly lumbar 3-4). After local routine disinfection and anesthesia, wear rubber gloves and slowly pierce in the direction of the spinous process with a No. 20 puncture needle (No. 21-22 for children). When the needle tip meets bone during the needle insertion process, the needle should be retracted under the skin. Perform the puncture after correcting the angle. When the needle is inserted about 4-6cm in adults (about 3-4cm in children), the dura mater can be penetrated to reach the subarachnoid space, and the needle core will flow out of the cerebrospinal fluid. After pressure measurement and slow discharge (no more than 2-3ml) ), then insert the needle core and pull out the puncture needle. Slightly pressurize the puncture point to stop bleeding, apply sterile gauze and fix it with tape. Lie supine for 4-6 hours postoperatively. If the initial pressure exceeds 2.94kPa (300mm water column), it is not suitable to discharge the liquid, just take the cerebrospinal fluid in the piezometer tube for cell count and protein quantification.
1. Instruct the patient to lie on his side on a rigid board with his back perpendicular to the bed surface, head forward and chest flexed, and hold the knees close to the abdomen with both hands, so that the torso is arched; or the assistant holds the patient’s head with one hand and the other hand opposite the surgeon Hold the lower limbs at the national nest and hold tightly to make the spine kyphosis to widen the intervertebral space and facilitate needle access.
2. Determine the puncture point. The intersection of the line of the posterior superior spine and the posterior midline is the puncture point. Generally, the 3-4th lumbar spinous process space is taken, and sometimes it can be performed in the previous or next lumbar space.
3. After routinely disinfecting the skin, wear sterile gloves and cover the hole, and use 2% lidocaine for local anesthesia from the skin to the intervertebral ligaments.
4. The surgeon fixes the skin of the puncture site with his left hand, and slowly punctures the puncture needle in the vertical north direction with his right hand. The depth of the needle is about 4-6cm for adults and 2-4cm for children. When the needle passes through the ligament and dura, the resistance suddenly disappears and a sense of frustration can be felt. At this time, the needle core can be slowly withdrawn (to prevent the cerebrospinal fluid from flowing out quickly and causing brain herniation), and the cerebrospinal fluid can be seen to flow out.
5. Connect the pressure measuring tube to measure the pressure before discharging. The pressure of the cerebrospinal fluid in the normal lateral position is 0.69-1.764kPa or 40-50 drops/min. If you know whether the subarachnoid space is obstructed, you can do the Queckenstedt test. That is, after the initial pressure is measured, the assistant first compresses one jugular vein for about 10 seconds, then presses the other side, and finally presses both jugular veins at the same time; when the jugular vein is compressed under normal conditions, the pressure of the cerebrospinal fluid immediately doubles rapidly. 10-20s after the compression is relieved, it quickly drops to the original level, which is called a negative obstruction test, indicating that the subarachnoid space is unobstructed. If the pressure of the cerebrospinal fluid cannot be increased after the jugular vein is compressed, the infarction test is positive, indicating that the subarachnoid space is completely blocked; if the pressure rises slowly after pressure is applied, and then slowly decreases after relaxation, it indicates incomplete blockage. Anyone with increased intracranial pressure is forbidden to do this test.
6. Remove the piezometer tube, collect 2-5ml of cerebrospinal fluid and submit it for inspection; if culture is needed, use aseptic method to retain specimens.
7. After the operation, insert the needle core and pull out the puncture needle together, cover it with sterile gauze, and fix it with tape.
8. After the operation, the patient goes to the pillow and prone (if it is difficult, then lie down) for 4-6 hours, so as to avoid postoperative headache with low intracranial pressure
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